Volume 2, Issue 4 (Autumn 2021)
J Vessel Circ 2021, 2(4): 195-200 |
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Faraji Shahrivar F, Khani S, Mehdipour A, Farahani H. The Effect of Therapeutic Hypothermia on Plasma Levels of Thyroid Hormones During Hemorrhagic Shock in Adult Male Wistar Rats. J Vessel Circ 2021; 2 (4) :195-200
URL: http://jvessels.muq.ac.ir/article-1-189-en.html
URL: http://jvessels.muq.ac.ir/article-1-189-en.html
1- Department of Physiology, School of Medicine, Iranshahr University of Medical Sciences, Iranshar, Iran., Department of Physiology, School of Medicine, Iranshahr University of Medical Sciences, Stree of Shahid Beheshti, Iranshahr, Iran.
2- Department of Physiology and Pharmacology, School of Medicine, Qom University of Medical Sciences, Qom, Iran, Department of physiology and pharmacology, School of Medicine, Educational building complex of Pardisan, Blv Al-Qadir- Qom University of Medical Sciences, Qom, Iran.
3- Department of Pediatric dentistry, School of dentistry, Saheli St, Qom University of medical sciences, Qom, Iran., Department of Pediatric dentistry, School of dentistry, Saheli St, Qom university of medical sciences, Qom, Iran.
4- Molecular & Cellular Research Center, School of Medicine, Qom University of Medical Sciences, Qom, Iran., Department of physiology and pharmacology, Molecular & Cellular Research Center, School of Medicine, Educational building complex of Pardisan, Blv Al-Qadir- Qom University of Medical Sciences, Qom, Iran.
2- Department of Physiology and Pharmacology, School of Medicine, Qom University of Medical Sciences, Qom, Iran, Department of physiology and pharmacology, School of Medicine, Educational building complex of Pardisan, Blv Al-Qadir- Qom University of Medical Sciences, Qom, Iran.
3- Department of Pediatric dentistry, School of dentistry, Saheli St, Qom University of medical sciences, Qom, Iran., Department of Pediatric dentistry, School of dentistry, Saheli St, Qom university of medical sciences, Qom, Iran.
4- Molecular & Cellular Research Center, School of Medicine, Qom University of Medical Sciences, Qom, Iran., Department of physiology and pharmacology, Molecular & Cellular Research Center, School of Medicine, Educational building complex of Pardisan, Blv Al-Qadir- Qom University of Medical Sciences, Qom, Iran.
Abstract: (918 Views)
Background and Aim: Hemorrhagic shock (HS) is one of the most important causes of death. In this study, we investigated the benefits of therapeutic hypothermia (32°C) during HS on blood pressure (BP) and the role of thyroid hormones during HS.
Materials and Methods: Twenty-four male Wistar rats were divided into two normothermic hemorrhagic (NH) and hypothermic hemorrhagic (HH) groups (32°C during shock); the animals were then anesthetized, a microcatheter was inserted into the femoral artery, and one into the femoral vein. The arterial samples were centrifuged, and plasma was isolated to measure thyroid hormones later. The microcatheter was fixed to a physiograph to record BP. Animals were exposed to HS for 90 minutes by withdrawing blood from the femoral vein and BP was assessed during HS.
Results: The BP of HH animals was significantly higher in most times of HS and at 40 minutes (56.8±4.2 mmHg) in comparison to NH rats (45.4±3.8 mmHg) (P<0.05). In addition, the amount of BP in HH animals at the end of the shock period at 90 minutes (63.8±5.5 mmHg) was significantly (P<0.001) higher in comparison with the NH groups (39±3.2 mmHg). Levels of thyroid hormones T4 and T3 at the end of shock were lower in the HH group compared to the NH group (P<0.001).
Conclusion: Therapeutic hypothermia indirectly reduces the level of thyroid hormones and directly reduces the metabolism of non-vital tissues, preserves blood in the central arteries, and increases BP.
Materials and Methods: Twenty-four male Wistar rats were divided into two normothermic hemorrhagic (NH) and hypothermic hemorrhagic (HH) groups (32°C during shock); the animals were then anesthetized, a microcatheter was inserted into the femoral artery, and one into the femoral vein. The arterial samples were centrifuged, and plasma was isolated to measure thyroid hormones later. The microcatheter was fixed to a physiograph to record BP. Animals were exposed to HS for 90 minutes by withdrawing blood from the femoral vein and BP was assessed during HS.
Results: The BP of HH animals was significantly higher in most times of HS and at 40 minutes (56.8±4.2 mmHg) in comparison to NH rats (45.4±3.8 mmHg) (P<0.05). In addition, the amount of BP in HH animals at the end of the shock period at 90 minutes (63.8±5.5 mmHg) was significantly (P<0.001) higher in comparison with the NH groups (39±3.2 mmHg). Levels of thyroid hormones T4 and T3 at the end of shock were lower in the HH group compared to the NH group (P<0.001).
Conclusion: Therapeutic hypothermia indirectly reduces the level of thyroid hormones and directly reduces the metabolism of non-vital tissues, preserves blood in the central arteries, and increases BP.
Type of Study: Research |
Subject:
vascular physiology
Received: 2021/12/22 | Accepted: 2022/01/2 | Published: 2021/12/1
Received: 2021/12/22 | Accepted: 2022/01/2 | Published: 2021/12/1
References
1. Krug EG, Sharma GK, Lozano R. The global burden of injuries. Am J Public Health. 2000; 90(4):523-6. [PMID] [DOI:10.2105/AJPH.90.4.523]
2. Saadat S, Yousefifard M, Asady H, Moghadas Jafari A, Fayaz M, Hosseini M. The most important causes of death in Iranian population: A retrospective cohort study. Emerg (Tehran). 2015; 3(1):16-21. [PMID]
3. Safarpour H, Khorasani-Zavareh D, Soori H, Bagheri Lankarani K, Ghomian Z, Mohammadi R. Vision zero: Evolution history and developing trend in road safety: A scoping review. Trauma Mon. 2020; 25(6):275-86. [DOI:10.30491/TM.2020.244740.1166]
4. Gutierrez G, Reines HD, Wulf-Gutierrez ME. Clinical review: Hemorrhagic shock. Crit Care. 2014; 8(5):373-81. [PMID] [DOI:10.1186/cc2851]
5. Bickell WH, Wall MJ Jr, Pepe PE, Martin RR, Ginger VF, Allen MK, et al. Immediate versus delayed fluid resuscitation for hypotensive patients with penetrating torso injuries. N Eng J Med. 1994; 331(17):1105-9. [PMID] [DOI:10.1056/NEJM199410273311701]
6. Radovsky A, Safar P, Sterz F, Leonov Y, Reich H, Kuboyama K. Regional prevalence and distribution of ischemic neurons in dog brains 96 hours after cardiac arrest of 0 to 20 minutes. Stroke. 1995; 26(11):2127-33; discussion 2133-4. [PMID] [DOI:10.1161/01.STR.26.11.2127]
7. Oliveira Filho LD, Saad KR, Saad PF, Koike MK, Silva SM, Montero EF. Effect of N-acetylcysteine in hearts of rats submitted to controlled hemorrhagic shock. Rev Bras Cir Cardiovasc. 2015; 30(2):173-81. [PMID] [PMCID] [DOI:10.5935/1678-9741.20140097]
8. Arrich J, Holzer M, Havel C, Müllner M, Herkner H. Hypothermia for neuroprotection in adults after cardiopulmonary resuscitation. Cochrane Database Syst Rev. 2016; 2(2):CD004128. [DOI:10.1002/14651858] [DOI:10.1002/14651858]
9. Varon J, Acosta P. Therapeutic hypothermia: Past, present, and future. Chest. 2008; 133(5):1267-74. [PMID] [DOI:10.1378/chest.07-2190]
10. Ringer SK, Clausen NG, Spielmann N, Weiss M. Effects of hypothermia and hypothermia combined with hypocapnia on cerebral tissue oxygenation in piglets. Paediatr Anaesth. 2020; 30(9):970-6. [PMID] [DOI:10.1111/pan.13943]
11. Storey KB, Storey JM. Tribute to P.L Lutz: Putting life on 'pause' - molecular regulation of hypometabolism. J Exp Biol. 2007; 210(Pt 10):1700-14. [PMID] [DOI:10.1242/jeb.02716]
12. Ireland S, Endacott R, Cameron P, Fitzgerald M, Paul E. The incidence and significance of accidental hypothermia in major trauma--a prospective observational study. Resuscitation. 2011; 82(3):300-6. [PMID] [DOI:10.1016/j.resuscitation.2010.10.016]
13. Mommsen P, Andruszkow H, Frömke C, Zeckey C, Wagner U, van Griensven M, et al. Effects of accidental hypothermia on posttraumatic complications and outcome in multiple trauma patients. Injury. 2013; 44(1):86-90. [PMID] [DOI:10.1016/j.injury.2011.10.013]
14. Knöller E, Stenzel T, Broeskamp F, Hornung R, Scheuerle A, McCook O, et al. Effects of hyperoxia and mild therapeutic hypothermia during resuscitation from porcine hemorrhagic shock. Crit Care Med. 2016; 44(5):264-77. [PMID] [DOI:10.1097/CCM.0000000000001412]
15. Song F, Guo C, Geng Y, Wu X, Fan W. Therapeutic time window and regulation of autophagy by mild hypothermia after intracerebral hemorrhage in rats. Brain Res. 2018; 1690:12-22. [PMID] [DOI:10.1016/j.brainres.2018.04.005]
16. Lilla N, Rinne C, Weiland J, Linsenmann T, Ernestus RI, Westermaier T. Early transient mild hypothermia attenuates neurologic deficits and brain damage after experimental subarachnoid hemorrhage in rats. World Neurosurg. 2018; 109:e88-98. [PMID] [DOI:10.1016/j.wneu.2017.09.109]
17. Jabbar A, Pingitore A, Pearce SH, Zaman A, Iervasi G, Razvi S. Thyroid hormone and cardiovascular disease. Nat Rev Cardiol. 2017; 14(1):39-55. [PMID] [DOI:10.1038/nrcardio.2016.174]
18. Meissner W, Krapp C, Kauf E, Dohrn B, Reinhart K. Thyroid hormone response to moderate hypothermia in severe brain injury. Intensive Care Med. 2003; 29(1):44-8. [PMID] [DOI:10.1007/s00134-002-1556-3]
19. Farahani H, Ghasemi A, Roghani M, Zahediasl S. Effect of neonatal hypothyroidism on carbohydrate metabolism, insulin secretion, and pancreatic islets morphology of adult male offspring in rats. J Endocrinol Invest. 2013; 36(1):44-9. [PMID]
20. Vitek V, Shatney CH, Lang DJ, Cowley RA. Thyroid hormone responses in hemorrhagic shock: Study in dogs and preliminary findings in humans. Surgery. 1983; 93(6):768-77. [PMID]
21. Shirpour A, Khameneh S, Zarghami N, Eskandari M. [The effect of hypothermia on thyroid function in rats (Persian)]. Iran J Endocrinol Metab. 2002; 4(1):35-40. [Link]
22. Meissner W, Krapp C, Kauf E, Dohrn B, Reinhart K. Thyroid hormone response to moderate hypothermia in severe brain injury. Intensive Care Med. 2003; 29(1):44-8. [PMID] [DOI:10.1007/s00134-002-1556-3]
23. Jiang S, He X, Wang J, Zhou G, Zhang M, Ba L, et al. Therapeutic mild hypothermia improves early outcomes in rabbits subjected to traumatic uncontrolled hemorrhagic shock. J Surg Res. 2013; 179(1):145-52. [PMID] [DOI:10.1016/j.jss.2012.09.024]
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